Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections.
Identifieur interne : 001504 ( Main/Exploration ); précédent : 001503; suivant : 001505Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections.
Auteurs : Salim Hage [Allemagne] ; Gertrud E. Morlock [Allemagne]Source :
- Journal of chromatography. A [ 1873-3778 ] ; 2017.
Descripteurs français
- KwdFr :
- Anti-infectieux (analyse), Anti-infectieux (composition chimique), Anti-infectieux (pharmacologie), Bactéries (effets des médicaments et des substances chimiques), Chromatographie sur couche mince (méthodes), Extraits de plantes (analyse), Extraits de plantes (composition chimique), Extraits de plantes (pharmacologie), Salicaceae (composition chimique).
- MESH :
- analyse : Anti-infectieux, Extraits de plantes.
- composition chimique : Anti-infectieux, Extraits de plantes, Salicaceae.
- effets des médicaments et des substances chimiques : Bactéries.
- méthodes : Chromatographie sur couche mince.
- pharmacologie : Anti-infectieux, Extraits de plantes.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Anti-Infective Agents, Plant Extracts.
- chemical , chemistry : Anti-Infective Agents, Plant Extracts.
- chemical , pharmacology : Anti-Infective Agents, Plant Extracts.
- chemistry : Salicaceae.
- drug effects : Bacteria.
- methods : Chromatography, Thin Layer.
Abstract
The buds of poplars (Populus L.) and willows (Salix L.), both from the same family (Salicaceae Mirbel), are increasingly used in gemmotherapy and importantly contribute to the production of the physiologically active propolis by European bee Apis mellifera L. In order to study their phenolic profiles, polar extracts of buds from P. nigra L. were compared to those of P. alba L. and S. alba L. through high-performance thin-layer chromatography (HPTLC). Five chemotypical patterns were distinguished after derivatisation with the Natural Product reagent and confirmed by principal component analysis. The HPTLC analysis was directly hyphenated to various microbiological and biochemical assays as well as spectrometric techniques, directly linking to active molecules in the chromatograms. At a glance, polyvalent compounds were evident when all derivatisation and activity assays, to which HPTLC was hyphenated at ease, were combined together. In Populus buds, at least three antimicrobial compound zones were detected using Aliivibrio fischeri and Bacillus subtilis bioassays, and one phyto-œstrogen with the planar yeast œstrogen screen. In all samples, several inhibitors of acetyl- and butyrylcholinesterase and rabbit liver esterase were detected. Hyphenation to high resolution mass spectrometry supported the assignment of bioactive compounds, as shown for chrysin as selective cholinesterase inhibitor as well as caffeic acid and galangin as antimicrobials in P. nigra and P. alba. This fast and cost-efficient method can be appropriately extended and applied to the botanical origin determination and quality control of bud extracts and propolis samples.
DOI: 10.1016/j.chroma.2017.02.019
PubMed: 28222858
Affiliations:
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Morlock</name><affiliation wicri:level="1"><nlm:affiliation>Chair of Food Sciences, Institute for Nutritional Science and Interdisciplinary Research Center (IFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: Gertrud.Morlock@uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Chair of Food Sciences, Institute for Nutritional Science and Interdisciplinary Research Center (IFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen</wicri:regionArea><wicri:noRegion>35392 Giessen</wicri:noRegion><wicri:noRegion>35392 Giessen</wicri:noRegion><wicri:noRegion>35392 Giessen</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of chromatography. 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In order to study their phenolic profiles, polar extracts of buds from P. nigra L. were compared to those of P. alba L. and S. alba L. through high-performance thin-layer chromatography (HPTLC). Five chemotypical patterns were distinguished after derivatisation with the Natural Product reagent and confirmed by principal component analysis. The HPTLC analysis was directly hyphenated to various microbiological and biochemical assays as well as spectrometric techniques, directly linking to active molecules in the chromatograms. At a glance, polyvalent compounds were evident when all derivatisation and activity assays, to which HPTLC was hyphenated at ease, were combined together. In Populus buds, at least three antimicrobial compound zones were detected using Aliivibrio fischeri and Bacillus subtilis bioassays, and one phyto-œstrogen with the planar yeast œstrogen screen. In all samples, several inhibitors of acetyl- and butyrylcholinesterase and rabbit liver esterase were detected. Hyphenation to high resolution mass spectrometry supported the assignment of bioactive compounds, as shown for chrysin as selective cholinesterase inhibitor as well as caffeic acid and galangin as antimicrobials in P. nigra and P. alba. This fast and cost-efficient method can be appropriately extended and applied to the botanical origin determination and quality control of bud extracts and propolis samples.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">28222858</PMID><DateCompleted><Year>2017</Year><Month>05</Month><Day>10</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3778</ISSN><JournalIssue CitedMedium="Internet"><Volume>1490</Volume><PubDate><Year>2017</Year><Month>Mar</Month><Day>24</Day></PubDate></JournalIssue><Title>Journal of chromatography. A</Title><ISOAbbreviation>J Chromatogr A</ISOAbbreviation></Journal><ArticleTitle>Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections.</ArticleTitle><Pagination><MedlinePgn>201-211</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0021-9673(17)30241-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chroma.2017.02.019</ELocationID><Abstract><AbstractText>The buds of poplars (Populus L.) and willows (Salix L.), both from the same family (Salicaceae Mirbel), are increasingly used in gemmotherapy and importantly contribute to the production of the physiologically active propolis by European bee Apis mellifera L. In order to study their phenolic profiles, polar extracts of buds from P. nigra L. were compared to those of P. alba L. and S. alba L. through high-performance thin-layer chromatography (HPTLC). Five chemotypical patterns were distinguished after derivatisation with the Natural Product reagent and confirmed by principal component analysis. The HPTLC analysis was directly hyphenated to various microbiological and biochemical assays as well as spectrometric techniques, directly linking to active molecules in the chromatograms. At a glance, polyvalent compounds were evident when all derivatisation and activity assays, to which HPTLC was hyphenated at ease, were combined together. In Populus buds, at least three antimicrobial compound zones were detected using Aliivibrio fischeri and Bacillus subtilis bioassays, and one phyto-œstrogen with the planar yeast œstrogen screen. In all samples, several inhibitors of acetyl- and butyrylcholinesterase and rabbit liver esterase were detected. Hyphenation to high resolution mass spectrometry supported the assignment of bioactive compounds, as shown for chrysin as selective cholinesterase inhibitor as well as caffeic acid and galangin as antimicrobials in P. nigra and P. alba. This fast and cost-efficient method can be appropriately extended and applied to the botanical origin determination and quality control of bud extracts and propolis samples.</AbstractText><CopyrightInformation>Copyright © 2017 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hage</LastName><ForeName>Salim</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Chair of Food Sciences, Institute for Nutritional Science and Interdisciplinary Research Center (IFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morlock</LastName><ForeName>Gertrud E</ForeName><Initials>GE</Initials><AffiliationInfo><Affiliation>Chair of Food Sciences, Institute for Nutritional Science and Interdisciplinary Research Center (IFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: Gertrud.Morlock@uni-giessen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>02</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Chromatogr A</MedlineTA><NlmUniqueID>9318488</NlmUniqueID><ISSNLinking>0021-9673</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000890">Anti-Infective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000890" MajorTopicYN="Y">Anti-Infective Agents</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002855" MajorTopicYN="N">Chromatography, Thin Layer</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="Y">Plant Extracts</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031308" MajorTopicYN="N">Salicaceae</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bioprofiling</Keyword><Keyword MajorTopicYN="N">Bud sap extracts</Keyword><Keyword MajorTopicYN="N">HPTLC–MS</Keyword><Keyword MajorTopicYN="N">Populus alba</Keyword><Keyword MajorTopicYN="N">Populus nigra</Keyword><Keyword MajorTopicYN="N">Salix alba</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>12</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>02</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>02</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28222858</ArticleId><ArticleId IdType="pii">S0021-9673(17)30241-8</ArticleId><ArticleId IdType="doi">10.1016/j.chroma.2017.02.019</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Hage, Salim" sort="Hage, Salim" uniqKey="Hage S" first="Salim" last="Hage">Salim Hage</name></noRegion><name sortKey="Morlock, Gertrud E" sort="Morlock, Gertrud E" uniqKey="Morlock G" first="Gertrud E" last="Morlock">Gertrud E. 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